Publisher: CRC; 1 Spi edition
Language: english
ISBN: 0849335280
Paperback: 408 pages
Data: December 13, 2006
Format: PDF
Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers- · Commercially available adjuvants designed for the production of antisera in the research setting · Methods for the production, purification, and characterization of antibodies · Practical guidance to researchers needing to modify antibodies · Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry · Methods for applying immunolabeling to the realm of electron microscopy (EM) · New antibody sources and new ways to use antobodies Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.
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